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Kutevedzera physiological mamiriro kunobatsira vaongorori kuwana simbi binders

Vatsvakurudzi vakagadzira nzira yekuziva mamorekuru madiki anosunga maion esimbi. Metal ions yakakosha mubiology. Asi kuziva kuti mamorekuru ndeapi-uye kunyanya mamorekuru madiki-aya maion esimbi anodyidzana nawo anogona kunetsa.

Kuparadzanisa mametabolites ekuongororwa, nzira dzakajairwa dzemetabolomics dzinoshandisa organic solvents uye yakaderera pHs, izvo zvinogona kuita kuti simbi yakaoma iparadzane. Pieter C. Dorrestein weUniversity of California San Diego nevamwe vaaishanda navo vaida kuchengeta macomplexes pamwe chete kuti aongororwe nekutevedzera mamiriro anowanikwa mumasero. Asi kana vakashandisa physiological mamiriro panguva yekuparadzaniswa kwemamorekuru, vangadai vakafanirwa kugadzirisa zvakare mamiriro ekuparadzanisa kune yega yega mamiriro emuviri wavaida kuyedza.

Pane kudaro, vatsvakurudzi vakagadzira nzira mbiri-nhanho iyo inotanga mamiriro ehupenyu pakati pezvakajairwa chromatographic separation uye mass spectrometric analysis (Nat. Chem. 2021, DOI: 10.1038 / s41557-021-00803-1). Chekutanga, vakapatsanura chidimbu chebiological vachishandisa yakajairika yepamusoro-inoshanda liquid chromatography. Vakabva vagadzirisa pH yekuyerera ichibuda mukromatographic column kutevedzera physiological mamiriro, vakawedzera simbi ions, uye vakaongorora musanganiswa ne mass spectrometry. Vakamhanyisa ongororo kaviri kuti vawane misa spectra yemamorekuru madiki ane uye asina simbi. Kuti vaone kuti ndeapi mamorekuru anosunga simbi, vakashandisa nzira yekombuta inoshandisa maumbirwo epamusoro-soro kuti vape hukama pakati pezvimiro zvemhando dzakasungwa uye dzisina kusungwa.

Imwe nzira yekuwedzera kutevedzera mamiriro emuviri, Dorrestein anoti, ingave yekuwedzera yakanyanya kuwanda kwemaion akadai sodium kana potasium uye yakaderera kudzika kwesimbi yekufarira. “Zvinova kuedza kwemakwikwi. Izvo zvinokuudza iwe, zvakanaka, iyi morekuru pasi peaya mamiriro ane hunyanzvi hwekusunga sodium nepotassium kana iyi simbi yakasarudzika yawakawedzera, ”anodaro Dorrestein. "Tinogona kupinza simbi dzakasiyana siyana panguva imwe chete, uye isu tinogona kunyatsonzwisisa zvatinoda uye kusarudza mumamiriro ezvinhu aya."

Muzvinyorwa zvetsika kubva kuEscherichia coli, vatsvakurudzi vakaona anozivikanwa iron-binding compounds seyersiniabactin uye aerobactin. Kana iri yersiniabactin, vakawana kuti inogona zvakare kusunga zinc.

Vatsvakurudzi vakacherechedza simbi-inosunga makomisheni mumasampuli akaoma seyakanyungudika organic zvinhu kubva munyanza. "Ndiyo imwe yemuenzaniso wakaoma kwazvo wandati ndambotarisa," Dorrestein anodaro. "Zvichida zvakaoma se, kana zvisina kuoma zvakanyanya kupfuura, crude oil." Iyo nzira yakaratidza domoic acid semhangura-inosunga morekuru uye yakataura kuti inosunga Cu2+ sedhiri.

"Maitiro ekuona mametabolites ese anosunga simbi mumuenzaniso anobatsira zvakanyanya nekuda kwekukosha kwebiological metal chelation," Oliver Baars, anodzidza metabolites inosunga simbi inogadzirwa nezvirimwa uye tupukanana paNorth Carolina State University, anonyora mu email.

"Dorrestein nevaanoshanda navo vanopa yakanakisa, inodiwa, kuyedza kuongorora zvirinani kuti basa remuviri remaoni esimbi muchitokisi ringave rini," Albert JR Heck, piyona muongororo yeruzhinji yeruzhinji paUtrecht University, anonyora mune email. "Inogoneka nhanho inotevera ingave yekubvisa mametabolites pasi pemamiriro ekunze kubva muchitokisi uye kupatsanura aya zvakare pasi pemamiriro ekunze, kuona kuti ndeapi mametabolites anotakura ayo endogenous cellular metal ions."

Nguva yekutumira: Zvita-23-2021